Sequencer Reference
This page provides guidance on sequencer-specific limitations, constraints, and capabilites.
Instrument | Run Setup | Run Monitoring | Data Uploaded to ICA | Automatic Secondary Analysis in ICA | Manual Secondary Analysis in ICA |
|---|---|---|---|---|---|
NovaSeq X Series | BaseSpace Run Planning, Sample Sheet | BaseSpace | BCLs, On-instrument analysis results | BCL Convert, DRAGEN Germline, DRAGEN Enrichment, DRAGEN RNA | DRAGEN Pipelines, Custom Pipelines |
NovaSeq6000/6000 Dx | BaseSpace Run Planning, Sample Sheet | BaseSpace | BCLs | BCL Convert, TSO 500 Tissue, ctDNA | DRAGEN Pipelines, Custom Pipelines |
NextSeq 1000/2000 | BaseSpace Run Planning, Sample Sheet | BaseSpace | BCLs | BCL Convert | DRAGEN Pipelines, Custom Pipelines |
NovaSeq 500/550/550 Dx | BaseSpace Run Planning, Sample Sheet | BaseSpace | BCLs | BCL Convert, TSO 500 Tissue, ctDNA | DRAGEN Pipelines, Custom Pipelines |
MiSeq/MiSeq Dx | Sample Sheet | BaseSpace | BCLs | BCL Convert | DRAGEN Pipelines, Custom Pipelines |
MiniSeq | Sample Sheet | BaseSpace | BCLs | BCL Convert | DRAGEN Pipelines, Custom Pipelines |
iSeq 100 | Sample Sheet | BaseSpace | BCLs | BCL Convert | DRAGEN Pipelines, Custom Pipelines |
- NextSeq 550 Dx and MiSeq Dx supported in RUO mode only.
- NovaSeq X series instruments support upload of on-instrument DRAGEN secondary analysis results to ICA. Must be configured during run setup.