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Sequencer Reference
This page provides guidance on sequencer-specific limitations, constraints, and capabilites.
Instrument | Run Setup | Run Monitoring | Data Uploaded to ICA | Automatic Secondary Analysis in ICA | Manual Secondary Analysis in ICA |
|---|---|---|---|---|---|
NovaSeq X Series | BaseSpace Run Planning, Sample Sheet | BaseSpace | BCLs, On-instrument analysis results | BCL Convert, DRAGEN Germline, DRAGEN Enrichment, DRAGEN RNA | DRAGEN Pipelines, Custom Pipelines |
NovaSeq6000/6000 Dx | BaseSpace Run Planning, Sample Sheet | BaseSpace | BCLs | BCL Convert, TSO 500 Tissue, ctDNA | DRAGEN Pipelines, Custom Pipelines |
NextSeq 1000/2000 | BaseSpace Run Planning, Sample Sheet | BaseSpace | BCLs | BCL Convert | DRAGEN Pipelines, Custom Pipelines |
NextSeq 500/550/550 Dx | BaseSpace Run Planning, Sample Sheet | BaseSpace | BCLs | BCL Convert, TSO 500 Tissue, ctDNA | DRAGEN Pipelines, Custom Pipelines |
MiSeq/MiSeq Dx | Sample Sheet | BaseSpace | BCLs | Not Supported (1) | DRAGEN Pipelines, Custom Pipelines, BCL Convert |
MiniSeq | Sample Sheet | BaseSpace | BCLs | BCL Convert | DRAGEN Pipelines, Custom Pipelines |
iSeq 100 | Sample Sheet | BaseSpace | BCLs | BCL Convert | DRAGEN Pipelines, Custom Pipelines |
- NextSeq 550 Dx and MiSeq Dx supported in RUO mode only.
- NovaSeq X series instruments support upload of on-instrument DRAGEN secondary analysis results to ICA. Must be configured during run setup.
(1) auto-launch from the instrument is currently not compatible with MiSeq due to the sample sheet constraints. The workaround is to upload your MiSeq run to BSSH via the BSSH CLI with an auto-launch configured v2 samplesheet.